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- Seq Only PDOs do not get BAM files in PDO_STAR5_Aux DM
- Plated sample in BSP_Plated_Samples is different than slxre_readgroup_metadata.sample_id
- Read group of the PDO Sample is missing in SLXRE2_RGHQS, most likely because there isn't a record in metrics.basecall_analysis for metrics_type Indexed or Unindexed.
- PM billed sample before PDOSTAR5 picked the most recent aggregation or before sample met full coverage
- Determine what is the root cause (using SUPPORT-9123 as an example)
Remedy:
- None
- Check if Mercury DWH and Mercury Run API still do not agree about the plated sample. If they don't - seek help from Mercury team. If they match, a refresh of the flowcell should correct it. BSP exported samples might be associated with the wrong PDO in BSP_Plated_samples because the mapping in BSP is not accurate. If it is important to fix the data , BSP team could help and then request a refresh for the affected plated sample as instructed in the recipe on this page to update BSP Plating DM. Finally, make sure to refresh in PDO Star5 if it won't happen automatically.
- If there is data for Indexed or Unindexed on metrics.basecall_analysis, then the flowcell needs to be refreshed in the datamarts. Otherwise, make ticket for the Pipeline and refer to PO-13231 as being the same issue.
- PDOs created within past year will get refreshed on Friday 2:55 am or Analytics manually refresh for faster data delivery. PDOs older than 1 year MUST be manually refreshed. See instructions for PDOSTAR5 refresh in Checking metadata changes.
- Steps to determine root cause of missing BAM file path
Info |
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To view Mercury Run API, add run_name to the end of this query (after 'runName='): https://mercury.broadinstitute.org:8443/Mercury/rest/IlluminaRun/query?runName=190725_SL-NVM_0088_AHKYJVDSXX |
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